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Graduate Pharmacy Aptitude Test (GPAT) 2018 Solved Paper

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Question : 2

Total: 125

Arrange the following steps in sequence of their order for production of recombinant Insulin:-
A. Fusion of A and B chains for disulphide bond.
B. Cynogen bromide treatment to remove methi onine and â galactosidase.
C. Introduction of A and B chain in the plasmid containing a galactosidase g ene.
D. Synthesis of A and B chain in E coli.

a⟶b⟶d⟶c
d⟶c⟶a⟶b
c⟶d⟶b⟶a
b⟶a⟶d⟶c

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