The various steps in DNA fingerprinting are: → DNA isolated from samples is digested with restriction endonucleases → The DNA molecules are separated by gel electrophoresis by Southern blot hybridization. → They are then transferred onto nitrocellulose or nylon membranes for immobilization, a process called Southern blot. → A radioactively labeled VNTR DNA probe containing the sequence of interest is then hybridized with the immobilized DNA on the membrane → Hybridization takes place with only those DNA molecules that contain a nucleotide sequence complementary to the sequence of the probe. Non-hybridized probe is then washed off the membrane. → The washed membrane is exposed to X-ray film to detect the presence of the radioactivity. Hence, it can be detected by autoradiography.