(d) : Secondary messengers are low-weight diffusible molecules that are used to relay signals within a cell. They are synthesized or released by specific enzymatic reactions, usually as a result of an external signal that is received by a transmembrane receptor. cAMP, cGMP and Ca2+ act as secondary messengers and are located within the cytoplasm. Sodium is an essential nutrient which helps to maintain blood volume and keeps nerves functioning.